Detection of human papilloma virus type 16 and 18 E6 gene in exfoliative cervical cell

观察宫颈脱落细胞标本是否能替代宫颈组织标本来检测HPV16、18型的感染。通过聚合酶链反应-限制性片段多态性分析，比较127例宫颈患者的单、双份宫颈脱落细胞和宫颈病变组织中HPV16、18型E6基因的检出率。发现单、双份宫颈脱落细胞标本中HPV16型E6基因的检出率分别为34.64%和40.73%；HPV18型的检出率为17.32和22.04%，存在差异。组织标本中HPV16、18型的检出率分别为41.73%和22.83%，与双份脱落细胞标本的检出率无明显差别。表明双份宫颈脱落细胞标本可替代宫颈组织标本检测患者宫颈组织中HPV16、18型感染情况。To observe possibility whether exfoliative cervical cell takes the place of cervical tissues for detection of human papilloma virus 16 and 18 types (HPV16 and HPV18). Polymerase chain reaction-restrictive fragment length polymorphism was used for analysis cervical tissues, single and double oxfoliative cervical cell samples of 127 cases of woman patients. Positive rates of HPV16 E6 Gene in single and double oxfoliative cervical cell are 34.64% and 40.73%, for HPV18 E6 gene 17.32% and 22.04%. In cervical tissues, 41.73% of HPV16 and 22.83% HPV18 special E6 genes was detected. There is no difference between cervical tissues and double oxfoliative cell samples. It is proved that double oxfoliative cervical cell sample can be used for detection of infection of HPV 16 and HPV 18 instead of cervical tissues.中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)0155-572