Expression of caspase 3 in the retina of inherited retinal degeneration

目的观察半胱氨酸天冬氨酸酶3(caspase 3)mRNA在RCS大鼠视网膜中的表达,分析caspase 3特异抑制剂Ac-DEVD-CHO对视细胞凋亡的影响.方法应用逆转录-多聚酶链反应(RT-PCR)检测RCS大鼠出生后不同时间视网膜中caspase3 mRNA的表达应用TUNEL方法检测玻璃体内注射Ac-DEVD-CHO后视细胞凋亡的改变.结果RCS大鼠出生后14天至60天视网膜caspase 3 mRNA均有表达,25天水平明显升高,达到高峰.SD大鼠视网膜15至60天的表达量无明显差异,与RCS大鼠14天水平接近.caspase 3特异抑制剂Ac-DEVD-CHO能显著抑制视细胞凋亡.结论caspase 3可能在RCS大鼠视网膜变性视细胞凋亡中起关键作用.Objective To observe the expression of caspase 3 mRNA in the retinas of RCS rats and study the effect of specific caspase 3 inhibitor on apoptosis of photoreceptor cells. Methods The expression of caspase 3 mRNA at different postnatal stages in the retina of RCS rats was studied by reverse transcription polymerase chain reaction (RT-PCR). TUNEL was performed to detect apoptosis after intra-vitreous injection of Ac-DEVD-CHO. Results The expression of caspase 3 mRNA was observed in the retinas of RCS rats from 14 to 60 days after birth. The peak expression was at 25th postnatal day and it was significantly higher than the expression of the other days. The expression of caspase 3 was also observed in the retina of SD rats from 15 to 60 postnatal days with no significant differences. Ac-DEVD-CHO treatment decreased TUNEL positive cells significantly. Conclusion caspase 3 may play a key role during the retina cell apoptosis in retinal degeneration of RCS rats.本课题受高等学校博士学科点专项科研基金资助中文核心期刊要目总览(PKU)04307-3112