Immunohistochemical expression of integrin αv,β5 and β3 in ovarian epithelial carcinoma:correlation with drug resistance of chemotherapy

目的探讨卵巢上皮性癌(卵巢癌)组织中整合素αv、β5和β3的表达与卵巢癌化疗耐药的关系及其临床意义.方法 1996年1月～2002年12月间符合纳入条件并完整随访的卵巢癌患者77例,根据临床治疗和随访结果分为化疗耐药组(26例)与非耐药(51例)组,采用免疫组化法测定卵巢癌组织中整合素αv、β5和β3的表达,并探讨其与化疗耐药的关系;采用多因素Logistic回归分析法探讨卵巢癌患者的年龄、手术病理分期、病理类型及分级、残留灶直径、化疗方案和整合素表达与卵巢癌化疗耐药的关系;同时对预后进行多因素的COX生存分析.结果整合素αv、β5的阳性表达率,耐药组分别为96%(25/26)和92%(24/26),均明显高于非耐药组的80%(41/51)和82%(42/51,P值分别为0.004和0.001);整合素β3的阳性表达率,耐药组为31%(8/26),非耐药组为35%(18/51),两组比较,差异无显著性(P＝0.668).多因素分析显示,整合素αv、β5表达与卵巢癌的手术病理分期均为与耐药(P值分别为0.014、0.030、0.042)及预后(P值分别为0.045、0.031、0.001)相关的独立危险因素.结论卵巢癌组织中整合素αv、β5的高表达是卵巢癌化疗耐药及预后的危险因素.To investigate the possible correlation between the expression of integrin alphav, beta5 and beta3 in tumor tissues and the response to chemotherapy and survival of patients with ovarian epithelial carcinoma.Seventy-seven patients with ovarian epithelial carcinoma from January 1996 to December 2002 were entered into the study. All subjects were matched up with the inclusion criteria and followed up till December 2003. Each patient received cytoreductive surgery and systematic chemotherapy. Patients were divided into drug resistant group (n = 26) and drug sensitive group (n = 51). The antigens of integrin alphav, beta5 and beta3 in ovarian tumour tissues were tested by immunohistochemistry. Logistic regression was employed to analyze the correlation between drug resistance and factors including patients' age, clinical stage, pathological degree and type of tumors, chemotherapy methods, operative results and the expression of integrin alphav, beta5 and beta3 in tumors. The disease prognosis was analyzed by multivariate COX regression.In the drug resistance group, the expressions of integrin alphav and beta5 were significantly higher than that in the drug sensitive group (P = 0.004 and 0.001). The expression of integrin beta3 was not statistically different in the two groups (P = 0.668). According to multivariate analysis, the expression of integrin alphav and beta5 and clinical stage were three independent factors correlated with drug resistance and disease prognosis for the patients with ovarian epithelial carcinoma (P = 0.014, 0.030, 0.042 for drug resistance; and P = 0.045, 0.031, 0.001 for prognosis respectively).The high expressions of integrin alphav and beta5 in ovarian epithelial carcinoma are risk factors for drug resistance and poor disease prognosis.PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)011750-7533