The pilot study on the correlation between human cytomegalovirus infection and the secretion of chemokines in the cultured human fibroblasts

目的 研究人巨细胞病毒(HCMV)对人胚肺成纤维细胞(HEL)分泌趋化因子白细胞介素-8(IL-8)及正常T细胞表达和分泌受活化调节的蛋白(RANTES)的影响,以探讨HCMV感染所致免疫病理改变的具体机制.方法 用RT-PCR法检测IL-8 mRNA和RANTES mRNA的表达,用双抗体夹心法(ELISA)测定IL-8和RANTES蛋白的分泌.结果 HEL细胞感染HCMV后,IL-8无论从mRNA水平还是蛋白水平,均随着感染时间的延长,表达逐渐增加,至感染72 h,IL-8 mRNA约为对照组的12倍,蛋白约为对照组的9倍.RANTES mRNA在感染后8 h可测出,24 h达到高峰,其后虽有下降,但仍维持较高水平;RANTES蛋白分泌在感染后24 h达到高峰,随后急剧下降,至72 h基本无表达.结论 HCMV感染HEL细胞诱导IL-8基因转录及IL-8蛋白分泌增加.HCMV感染HEL细胞可下调RANTES的分泌.Objective To investigate the effect of HCMV infection on the expression of interleukin-8(IL-8)and regulated on activation,normal T expressed and secreted(RANTES)so as to explore the possible mechanism of the immune-pathological changes caused by HCMV infection.Methods Expression of both IL-8 and RANTES mRNA was detected by RT-PCR.Secretion of IL-8 and RANTES protein was quantitated by enzyme linked immune-sorerbant assay(ELISA).Results After HCMV infection.the expression of IL-8 ia HEL cells was foand to be increased gradually concomitant with the prolonged infection time at both the transcriptional level and the protein level.By the time of 72h.p.i.as compared with mock-infected cells.IL-8 mRNA expression was increased up to 12-fold and IL-8 protein up to 9-fold in HCMV-infected cells.The expression of RANTES mRNA was occurred at 8h.P.i,with a maximum at 24 h.P.i.since then it remained relatively high level.The expression of RANTES protein peaked at 24 h.P.i.then dropped sharply and was almost undetectable by the time at 72 h.P.i.Conclusion HCMV infection of HEL cells may ieduce the transcription of IL-8 gene as well as the production of IL-8 protein.HCMV may down-regulate extra-cellular production of RAMTES protein.高等学校博士点新教师基金PubMed中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)05325-3272