The polymerization and denaturation of brain tubulin and the effect of taxol by differential scanning calorimetry

The thermal properties of porcine brain tubulin polymerization and denaturation and the efftcts of taxol were studied from 4 ℃ to 115 ℃ with a new highly-sensitive differential scanming calorimeter (Micro DSC Ⅲ, Setaram,France). The DSC results indicated that microtubule polymerization can proceed ...The thermal properties of porcine brain tubulin polymerization and denaturation and the effects of taxol were studied from 4 degrees C to 115 degrees C with a new highly-sensitive differential scanning calorimeter (Micro DSC III, Setaram, France). The DSC results indicated that microtubule polymerization can proceed via an endothermic process and the taxol could alter the process. A large aggregation peak was found at about 85 degrees C in the course of the denaturation and it disappeared with adding taxol. The heat flow oscillation was observed during the controlled-temperature experiments and taxol could stabilize the structure of the microtubules so that the oscillation disappeared.SCI(E)中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)102182-1851