Rapid and Direct Analysis of Active Ingredients in Tablets Using Atmospheric Pressure Laser Desorption Ionization Mass Spectrometry

采用大气压激光解吸附离子化质谱(APLDI-MS)技术对多种药片的有效成分进行了快速实时分析.在不进行任何样品处理的条件下,含有一种或多种有效成分的药片,均可通过APLDI-MS分析直接得到其有效成分的质谱信号,并且可通过二级质谱对它们进行结构确认.APLDI在离子化过程中不需加载高电压,也不使用溶剂或气体辅助离子化；同时由于所使用的近红外脉冲激光具有较强的穿透性,因此对于有薄衣包覆的药片也可以直接进行检测.本方法具有简便、快速、准确的优势,在药物高通量筛查、质量控制、打击药品违法添加等领域具有广阔的应用前景.An atmospheric pressure laser desorption ionization mass spectrometric (APLDI-MS) method has been developed for the fast and direct screening of active ingredients in drug tablets. Tablets containing one active ingredient as well as more than one active ingredient have been directly analyzed by APLDI-MS without any sample preparation, and the identities of the active ingredients are further confirmed by tandem MS. The ionization process of APLDI does not need high voltage or require auxiliary solvent or gas, and the good penetration depth of the near-infrared pulse laser enables APLDI to direct analyze tablets with a coated film. This simple, rapid and direct APLDI-MS method is useful for high-throughput screening of pharmaceuticals and for on-line quality control in pharmaceutical industry.国家自然科学基金http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318745100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701SCI(E)中国科学引文数据库(CSCD)33366-3704