EFFECT OF RARE EARTH IONS Eu3+、Tb3+ ON THE SECONDARY STRUCTURE OF CALMODULINS

The secondary structure of four kinds of Calmodulins (CaM), i.e. Brassica Campestris Pollen Calmodulin (CaM-BCP), Bovine Brain Calmodulin (CaM-BB), Earthworm Calcium Binding Protein (CaM-CBP) and Earthworm New Calcium Binding Protein (CaM-NCBP) in thin film were determined through the technique of FT-IR spectroscopic method and curve-fitting. The changes in the secondary structure of Calmodulin upon the addition of Ca2+, Eu3+, Tb3+ and the activity of PDE enzyme were also measured, from which the effect of rare earth ions on the conformation of Calmodulins were discussed.The secondary structure of four kinds of Calmodulins (CaM), i.e. Brassica Campestris Pollen Calmodulin (CaM-BCP), Bovine Brain Calmodulin (CaM-BB), Earthworm Calcium Binding Protein (CaM-CBP) and Earthworm New Calcium Binding Protein (CaM-NCBP) in thin film were determined through the technique of FT-IR spectroscopic method and curve-fitting. The changes in the secondary structure of Calmodulin upon the addition of Ca2+, Eu3+, Tb3+ and the activity of PDE enzyme were also measured, from which the effect of rare earth ions on the conformation of Calmodulins were discussed.中国科学院资助项目; 高等学校博士学科点专项科研项目http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000084248100018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701SCI(E)中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)44509-5121