Mycoplasma infection in human gastriointestinal carcinoma tissues

目的检测胃肠肿瘤组织支原体的感染,探讨支原体感染与胃肠肿瘤发生的相关性.方法抗肿瘤单克隆抗体(McAb)PD4的抗原P40通过测序,Hoechst22358荧光染色支原体及支原体DNA扩增等方法被证明为猪鼻支原体.利用PD4单抗通过免疫组织化学,检测石蜡包埋的人胃肠道的肿瘤组织的支原体感染,并用支原体特异性引物对石蜡包埋组织通过PCR扩增支原体DNA片段,进行进一步验证.以胃炎、胃溃疡、胃肠上皮化生及腺瘤性息肉标本为对照.结果在检测的标本中,56%(50/90)的胃癌组织有支原体感染,感染随分化程度的降低而降低,在对照的胃炎组的感染率为28%(13/47),肠上皮化生组为37%(18/49),胃溃疡组为30%(14/46),与胃癌组比较差异均有显著意义(P<0.05). 55.1%(32/58)的肠癌组织有支原体感染,感染随分化程度的降低而降低,对照的肠腺瘤性息肉组感染率为20.4%(10/49例),与肠癌比较差异有显著意义(P<0.005).结论胃肠道肿瘤组织中支原体感染较非肿瘤疾病有显著提高,提示支原体感染与肿瘤发生可能有一定的相关性.To explore the association between the carcinoma and mycoplasma infection by immunohistochemistry.Antigen of anti-tumor monoclonal antibody PD4 was identified as mycoplasma hyorhinis. Using immunohistochemistry to study archived paraffin-embedded gastriointestinal tract carcinomas tissue. The PCR was applied to amplify the mycoplasma DNA for confirming immunohistochemistry results.It was showed that mycoplasma was present in 56% (50/90 cases) of gastric carcinoma. The cancer tissues with high differentiation had a higher mycoplasma infection ratio than that of low differentiation cancer tissues. (P < 0.05). In control cases, mycoplasma infection was 28% (18/49) in chronic superficial gastritis, 30% (14/46) in gastric ulcer and 37% (18/49) in intestinal metaplasia of the stomach. Mycoplasma infection was 55.1% (32/58) in colon carcinoma and 20.9% (10/49) in adenomarous polyp (P < 0.005). It seems that colon carcinoma tissues with slight pathological grade had a higher percent of mycoplasma infection than that of cancer tissues with moderate and heavy pathological grade (P < 0.05).The high infection of mycoplasmas in carcinoma tissues suggest an association between mycoplasma and cancer. The mechanism involved in oncogenesis by mycoplasmas remains to be elucidated.国家高技术研究发展计划(863计划); 国家自然科学基金; 北京市自然科学基金PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)010601-6048