Ninety-day Rat Feeding Study of 5' -Nucleotide

目的:评价5'-核苷酸的安全性.方法:采用大鼠90d喂养实验,96只SD大鼠随机分为6组,每组16只,雌雄各半,分别喂饲添加不同剂量核苷酸(0、0.01、0.04、0.16、0.64、1.28g/kg bw)的饲料.观察大鼠体质量、进食量、食物利用率、血压、血液常规、生化指标及病理结果的变化.结果:5'-核苷酸组与对照组相比,大鼠的总体质量增大、总进食量、总食物利用率、血压均没有明显差异(P>0.05);雄性大鼠前4周的体质量明显增高(P<0.05),雄性0.04g/kg bw组第4周、雌性0.16g/kgbw组第1周和0.01g/kg bw组第2周的食物利用率显著增高(P<0.05);雄性大鼠的白细胞、红细胞、血红蛋白、血小板、总蛋白含量、白蛋白、甘油三酯与对照组比较有统计学差异,但均未超出正常范围.镜下观察各组大鼠的脏器未见有意义的病理学改变.结论:在本实验的剂量范围内添加5'-核苷酸是安全的.Objective: To explore the safety of 5'-nucleotide in rots. Methods: Totally 96 rats were randomly divided into 6 groups and fed diet containing 5-'nucleotide at doses of 0, 0.01, 0.04, 0.16, 0.64 g/kg bw and 1.28 g/kg bw for 90 consecutive days. The changes of body weight, dietary intake amount, food utilization rate, blood pressure, blood routine, biochemical parameters, and pathological results of the rats were recorded at intervals of one week during the administration of 5' -nucleotide. Results: No obvious difference in total body weight gain, total dietary intake amount, food utilization rate and systolic blood pressure was observed between 5'-nucleotide treatment groups and control group. However, compared with the rats in control group, body weights of male rats in nucleotide treatment group exhibited a significant increase in the first 4 weeks (P < 0.05). Similarly, total food utilization rates of the rats treated with 5' -nucleotide at doses of 0.01, 0.04 g/kg bw and 0.16 g/kg bw exhibited significantly higher than that of control group in the first 2 weeks (P <0.05). In addition, compared with the control group, white blood cells, red blood cells, hemoglobin, platelets, total protein, albumin, alanine transaminasse, triglyceride of male rats also revealed a significant difference. However, all of these values were still in the normal range. No histopathological change was observed in kidney and other organs or tissues. Conclusion: It is safety to rats fed 5' -nucleotide at the dose of 0.01 - 1.28 g/kg bw.中文核心期刊要目总览(PKU)中国科技核心期刊(ISTIC)中国科学引文数据库(CSCD)05283-2873