Effects of the inhalable particle (PM10) on secretion of inflammatory factors in human lung fibroblasts and mouse alveolar macrophage cell

目的 探讨大气可吸入颗粒物(PM10)对小鼠肺泡巨噬细胞(RAW264.7)和人肺成纤维细胞(HLF)分泌肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)和白细胞介素8(IL-8)的影响.方法 颗粒物样品采自北京市城区采暖期.两种染毒途径:(1)不同剂量的PM10直接对HLF染毒;(2)PM10首先作用于RAW264.7细胞,染毒一定时间后的细胞上清液再作用于HLF细胞.采用四甲基偶氮唑盐微量酶反应比色(MTT)法测定细胞毒性;放射免疫法测定炎性因子TNF-α、IL-6和IL-8.结果 PM10作用24h后,表现为低剂量时刺激细胞增殖,而高剂量时抑制细胞增殖,细胞存活率随浓度增加而降低(P＜0.01);PM10能刺激HLF分泌炎性因子TNF-α、IL-6和IL-8,且有剂量-反应关系(P＜0.05);巨噬细胞上清液亦能刺激HLF产生TNF-α、IL-6和IL-8,且其产生量高于PM10作用于HLF产生的量(P＜0.05).结论 大气可吸入颗粒物PM10对HLF有一定毒性作用;PM10染毒24小时后能诱导人肺成纤维细胞分泌炎性因子TNF-α、IL-6和IL-8;巨噬细胞上清液亦能刺激人肺成纤维细胞释放上述三种炎性因子,其产生量高于PM10.To study the effects of PM10 and the concentrated culture suspension of RAW264.7 cells treated with PM10 on the secretion of tumor necrosis factor-alpha (TNF-alpha), interleukin-6(IL-6) and interleukin-8(IL-8) in the human lung fibroblasts( HLF).PM10 were collected during heating period in the urban area of Beijing. HLF and mouse alveolar macrophage cell (RAW264.7) were used. The two ways of exposed cells to PM10 were used: (1) HLF were treated with the different concentration of PM10 for 24h. (2) HLF were stimulated by the added the suspension of RAW264.7 cell exposed to different concentrations of PM10. Cytotoxicity of PM10 was measured by MTT assay. The levels of inflammatory cytokines TNF-alpha, IL-6 and IL-8 were determined by the radioimmunity assay.After treated for 24h, PM10 showed the cytotoxicity in HLF and RAW264.7 cells, which was characterized by increase of the viability of cells at low concentration of PM10, and decrease at high doses. PM10 induced the secretion of TNF-alpha, IL-6 and IL-8 in HLF in a dose-dependent manner. The concentrated culture medium of RAW264.7 cells treated with PM10 also stimulated the secretion of TNF-alpha, IL-6 and IL-8 in HLF.PM10 is cytotoxic to HLF and RAW264.7 cells. PM10 induced the secretion of TNF-alpha, IL-6 and IL-8 in HLF. This effect was also observed by treatment of HLF with the concentrated culture medium of RAW264.7 cell exposed to PM10.北京市自然科学基金PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)05557-5603