Study on the developmental toxicity of Bisphenol A by using micromass culture in vitro

采用微团培养观察了不同浓度的双酚A(0～50mg/L)对体外培养的Wistar大鼠胚胎肢芽细胞增殖和分化的影响,旨在了解双酚A的体外发育毒性特点及其可能的作用机制.结果显示:双酚A对体外培养的Wistar大鼠胚胎肢芽细胞的增殖和分化均有抑制作用,表现为染毒组肢芽细胞集落形成数量减少,细胞毒性试验吸光度值下降,并呈现出明显的剂量-反应关系.双酚A对肢芽细胞的50%增殖抑制浓度(IP50)和50%分化抑制浓度(ID50)分别为38.74mg/L 和27.93mg/L,IP50/ID50=1.39.按照Renault等人推荐的体外致畸分类标准,双酚A属于阳性致畸物,对肢芽细胞的分化和增殖有特异性抑制作用.The micromass culture of Wistar rat embryo limb bud cells was used to investigate the characteristic of developmental toxicity of Bisphenol A (BPA) and its mechanism in vitro. The results showed that BPA inhibited both proliferation and differentiation of rat limb bud cells in vitro. The high level of BPA appeared to be cytotoxic to Wistar rat embryo limb bud cells in culture and inhibited the clone formation with dose-response relationship. The concentration of BPA for IP50 and ID50 were 38.74 mg/L and 27.93 mg/L respectively. According to Renaults' teratogenic criteria, BPA belonged to a positive teratogen. And the data suggested that the specific inhibition of cell proliferation and differentiation might be one of the mechanisms of high level BPA.国家重点基础研究发展计划(973计划); 国家自然科学基金PubMed中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)03178-179,1833