Aktivitas Sitotoksik Ekstrak Etanol dan Fraksi-Fraksi Daun Selaginella willdenowii terhadap Sel Kanker Payudara T47D

Breast cancer is a type of cancer with the highest percentage of new cases and death in Indonesia. Cancer treatment has been causing adverse effects, thus triggering the development of alternative medicines derived from nature or plants as anticancer therapy. Selaginella willdenowii is one of plants that has anticancer activity. The purposes of this research were to investigate cytotoxic activity of ethanol extract and fraction of S. willdenowii leaves against T47D breast cancer cell and to determine the class of compound in the active fraction. The leaf extract of S. willdenowii was obtained by maceration method using 96% ethanol. The cytotoxic assay was performed based on MTT assay method. The group of compounds contained in ethyl acetate fraction were identified using Thin Layer Chromatography (TLC) method. The result of cytotoxic test showed that the ethyl acetate fraction of S. willdenowii leaf have weak cytotoxic activity on T47D cancer cell with IC50 value of 259,380 μg/mL meanwhile ethanol extract, n-hexane fraction, and ethanol-water fraction did not have cytotoxic activity against breast cancer cells T47D. Doxorubicin as positive control has IC50 value of 39,770 μg/mL. The results of phytochemical analysis showed that ethyl acetate fraction of S. willdenowii leaves contains alkaloids, flavonoids, and terpenoids