SPLIT-STED Imaging of Nuclear Structures

The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated emission depletion (STED), excited fluorophores located on the periphery of the Point Spread Function (PSF) are completely switched off by a doughnut-shaped beam, producing an effective PSF of smaller size