Embryonic stem cells (ESCs) can self-renew or differentiate into all cell types, a phenomenon known as pluripotency. Distinct pluripotent states have been described, termed "naïve" and "primed" pluripotency. The mechanisms that control naïve-primed transition are poorly understood. In particular, we remain poorly informed about protein kinases that specify naïve and primed pluripotent states, despite increasing availability of high-quality tool compounds to probe kinase function. Here, we describe a scalable platform to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition in mouse ESCs. This approach utilizes simple cell culture conditions and standard reagents, materials and equipment to ...
Pluripotent stem cells are classified into naïve and primed based on their growth characteristics in...
Mouse and human embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts how...
The embryonic stem cells (ESCs) are pluripotent, self-renewing cells that are able to differentiate ...
Embryonic stem cells (ESCs) can self-renew or differentiate into all cell types, a phenomenon known ...
Naive human embryonic stem cells (hESCs) have been isolated that more closely resemble the pre-impla...
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gs...
Embryonic Stem Cells (ESCs) can self renew or differentiate into all cell types, a phenomenon known ...
Post-translational modification of proteins by phosphorylation plays a key role in regulating all as...
Embryonic stem cells (ESCs) of mice and humans have distinct molecular and biological characteristic...
Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) represent opposite ends o...
Background : The requirement of the Mek1 inhibitor (iMek1) during naïve pluripotency maintenance res...
Pluripotent cells can be subdivided into two distinct states, the naïve and the primed state, the la...
Historically, primordial germ cells (PGCs) have been a good model to study pluripotency. Despite the...
AbstractUnderstanding the complex mechanisms that govern the fate decisions of human embryonic stem ...
Here we utilized the chromatin in vivo assay (CiA) mouse platform to directly examine the epigenetic...
Pluripotent stem cells are classified into naïve and primed based on their growth characteristics in...
Mouse and human embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts how...
The embryonic stem cells (ESCs) are pluripotent, self-renewing cells that are able to differentiate ...
Embryonic stem cells (ESCs) can self-renew or differentiate into all cell types, a phenomenon known ...
Naive human embryonic stem cells (hESCs) have been isolated that more closely resemble the pre-impla...
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gs...
Embryonic Stem Cells (ESCs) can self renew or differentiate into all cell types, a phenomenon known ...
Post-translational modification of proteins by phosphorylation plays a key role in regulating all as...
Embryonic stem cells (ESCs) of mice and humans have distinct molecular and biological characteristic...
Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) represent opposite ends o...
Background : The requirement of the Mek1 inhibitor (iMek1) during naïve pluripotency maintenance res...
Pluripotent cells can be subdivided into two distinct states, the naïve and the primed state, the la...
Historically, primordial germ cells (PGCs) have been a good model to study pluripotency. Despite the...
AbstractUnderstanding the complex mechanisms that govern the fate decisions of human embryonic stem ...
Here we utilized the chromatin in vivo assay (CiA) mouse platform to directly examine the epigenetic...
Pluripotent stem cells are classified into naïve and primed based on their growth characteristics in...
Mouse and human embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts how...
The embryonic stem cells (ESCs) are pluripotent, self-renewing cells that are able to differentiate ...