Endosome transport by transcytosis is the primary mechanism by which proteins and other large cargo traverse epithelial barriers in normal tissue. Transcytosis is also essential for establishing and maintaining membrane polarity in epithelia and other polarized cells. To identify novel components of this pathway, we conducted a high-throughput RNA interference screen for factors necessary for the bidirectional transcytosis of IgG by the Fcγ receptor FcRn. This screen identified 23 genes whose suppression resulted in a reproducible decrease in FcRn-mediated transcytosis. Pulse-chase kinetic transport assays on four of the top-ranking genes (EXOC2, EXOC7, PARD6B, and LEPROT) revealed distinct effects on the apical and basolateral recycling an...
The establishment and maintenance of polarized plasma membrane domains is essential for cellular fun...
Recent evidence has established that extracellular vesicles (EVs), including exosomes and microvesic...
Epithelial tissues which line the body cavities and tubules typically face two environments that are...
The human body is composed of hundreds of specialized cell types, each fulfilling distinct functions...
International audiencePolarized epithelial cells contain apical and basolateral surfaces with distin...
The Fc receptor FcRn traffics immunoglobulin G (IgG) in both directions across polarized epithelial ...
Protein delivery across polarized epithelia is controlled by receptor-mediated transcytosis. Many st...
The transcytotic pathway followed by the polymeric IgA receptor (pIgR) carrying its bound ligand (dI...
The octameric exocyst complex is associated with the junctional complex and recycling endosomes, and...
The endocytic and postendocytic trafficking pathways in polarized epithelial cells was examined. Fir...
Efficient and selective delivery and retrieval of cargo to polarized plasma membrane domains are ess...
Recycling endosomes have taken central stage in the intracellular sorting and polarized trafficking ...
The small GTPase Rab17 is restricted to epithelial cells and its expression is induced during cell p...
The endocytic and exocytic system is important for cells to communicate with their surroundings. For...
International audienceApical polarity of cystic fibrosis transmembrane conductance regulator (CFTR) ...
The establishment and maintenance of polarized plasma membrane domains is essential for cellular fun...
Recent evidence has established that extracellular vesicles (EVs), including exosomes and microvesic...
Epithelial tissues which line the body cavities and tubules typically face two environments that are...
The human body is composed of hundreds of specialized cell types, each fulfilling distinct functions...
International audiencePolarized epithelial cells contain apical and basolateral surfaces with distin...
The Fc receptor FcRn traffics immunoglobulin G (IgG) in both directions across polarized epithelial ...
Protein delivery across polarized epithelia is controlled by receptor-mediated transcytosis. Many st...
The transcytotic pathway followed by the polymeric IgA receptor (pIgR) carrying its bound ligand (dI...
The octameric exocyst complex is associated with the junctional complex and recycling endosomes, and...
The endocytic and postendocytic trafficking pathways in polarized epithelial cells was examined. Fir...
Efficient and selective delivery and retrieval of cargo to polarized plasma membrane domains are ess...
Recycling endosomes have taken central stage in the intracellular sorting and polarized trafficking ...
The small GTPase Rab17 is restricted to epithelial cells and its expression is induced during cell p...
The endocytic and exocytic system is important for cells to communicate with their surroundings. For...
International audienceApical polarity of cystic fibrosis transmembrane conductance regulator (CFTR) ...
The establishment and maintenance of polarized plasma membrane domains is essential for cellular fun...
Recent evidence has established that extracellular vesicles (EVs), including exosomes and microvesic...
Epithelial tissues which line the body cavities and tubules typically face two environments that are...