Despite many methodological advances that have facilitated investigation of Mycobacterium tuberculosis pathogenesis, analysis of essential gene function in this slow-growing pathogen remains difficult. Here, we describe an optimized CRISPR-based method to inhibit expression of essential genes based on the inducible expression of an enzymatically inactive Cas9 protein together with gene-specific guide RNAs (CRISPR interference). Using this system to target several essential genes of M. tuberculosis, we achieved marked inhibition of gene expression resulting in growth inhibition, changes in susceptibility to small molecule inhibitors and disruption of normal cell morphology. Analysis of expression of genes containing sequences similar to thos...
Stewart Cole and colleagues determined the complete genome sequence of Mycobacterium tuberculosis (M...
DNA damage repair mechanisms are critical to the adaptive evolution of Mycobacterium tuberculosis as...
International audienceCRISPR-cas locus in M. tuberculosis has long been considered as a mere bar cod...
Development of new drug regimens that allow rapid, sterilizing treatment of tuberculosis has been li...
The ability to genetically engineer pathogenic mycobacteria has increased significantly over the las...
Royal Society Challenge Grant [REF: CH160014 to M.F.W.]; Biotechnology and Biological Sciences Resea...
International audienceClustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems...
The global health impact of M. tuberculosis necessitates the development of new genetic tools for it...
Existing antibiotics are inadequate to defeat tuberculosis (TB), a leading cause of death worldwide....
© 2021 Faulkner, Cox, Goh, van Bohemen, Gibson, Liebster, Wren, Willcocks and Kendall. This is an op...
ABSTRACT New tools for genetic manipulation of Mycobacterium tuberculosis are needed for the develop...
Mycobacterium tuberculosis is a major human pathogen. The complete genome sequence of M. tuberculosi...
Mycobacterium tuberculosis (M. tuberculosis) survives and multiplies inside human macrophages by sub...
Mycobacterium tuberculosis (Mtb) represents one of the most persistent bacterial threats to human he...
Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences...
Stewart Cole and colleagues determined the complete genome sequence of Mycobacterium tuberculosis (M...
DNA damage repair mechanisms are critical to the adaptive evolution of Mycobacterium tuberculosis as...
International audienceCRISPR-cas locus in M. tuberculosis has long been considered as a mere bar cod...
Development of new drug regimens that allow rapid, sterilizing treatment of tuberculosis has been li...
The ability to genetically engineer pathogenic mycobacteria has increased significantly over the las...
Royal Society Challenge Grant [REF: CH160014 to M.F.W.]; Biotechnology and Biological Sciences Resea...
International audienceClustered Regularly Interspaced Short Palindromic repeats (CRISPR)-Cas systems...
The global health impact of M. tuberculosis necessitates the development of new genetic tools for it...
Existing antibiotics are inadequate to defeat tuberculosis (TB), a leading cause of death worldwide....
© 2021 Faulkner, Cox, Goh, van Bohemen, Gibson, Liebster, Wren, Willcocks and Kendall. This is an op...
ABSTRACT New tools for genetic manipulation of Mycobacterium tuberculosis are needed for the develop...
Mycobacterium tuberculosis is a major human pathogen. The complete genome sequence of M. tuberculosi...
Mycobacterium tuberculosis (M. tuberculosis) survives and multiplies inside human macrophages by sub...
Mycobacterium tuberculosis (Mtb) represents one of the most persistent bacterial threats to human he...
Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences...
Stewart Cole and colleagues determined the complete genome sequence of Mycobacterium tuberculosis (M...
DNA damage repair mechanisms are critical to the adaptive evolution of Mycobacterium tuberculosis as...
International audienceCRISPR-cas locus in M. tuberculosis has long been considered as a mere bar cod...