Antimicrobial 3D Porous Scaffolds Prepared by Additive Manufacturing and Breath Figures

We describe herein a novel strategy for the fabrication of efficient 3D printed antibacterial scaffolds. For this purpose, both the surface topography as well as the chemical composition of 3D scaffolds fabricated by additive manufacturing were modified. The scaffolds were fabricated by fused deposition modeling (FDM) using high-impact polystyrene (HIPS) filaments. The surface of the objects was then topographically modified providing materials with porous surfaces by means of the Breath Figures approach. The strategy involves the immersion of the scaffold in a polymer solution during a precise period of time. This approach permitted the modification of the pore size varying the immersion time as well as the solution concentration. Moreover, by using polymer blend solutions of polystyrene and polystyrene-b-poly(acrylic acid) (PS-b-PAA) and a quaternized polystyrene-b-poly(dimethylaminoethyl methacrylate) (PS-b-PDMAEMAQ), the scaffolds were simultaneously chemically modified. The surfaces were characterized by scanning electron microscopy and infrared spectroscopy. Finally, the biological response toward bacteria was explored. Porous surfaces prepared using quaternized PDMAEMA as well as those prepared using PAA confer antimicrobial activity to the films, i.e., were able to kill on contact Staphylococcus aureus employed as model bacteria.We gratefully acknowledge support from the Consejo Superior de Investigaciones Cienti ́ fi cas (CSIC). Equally, this work was fi nancially supported by the Ministerio de Economi ́ ay Competitividad (MINECO) through MAT2016-78437-R (AEI/FEDER, UE), BIO2012-34835 and BIO2017-77367-C2- 1R projects. Nelson Vargas Alfredo thanks the support for postdoctoral research from the CONACYT (CN: 274411)Peer Reviewe