Rapid detection of sepsis witha point-of care and label free microarray platform

Resumen del póster presentado al VIII International Congress on Analytical Nanoscience and Nanotechnology, celebrado en Barcelona (España) del 3 al 5 de julio de 2017.-- et al.Sepsis is a potentially fatal whole-body inflammatory reaction caused by severe infection. It reaches a 35% mortality rate and accounts for more than 7 million deaths/year. The early diagnosis and subsequent stratification of its severity is very important and helps start timely and specific treatments. More than 170 biomarkers are described for sepsis but not all of them have a sufficient specificity or sensitivity to be routinely used in clinical practice. Within the frame of a Horizon 2020 European project (RAIS, www.rais-project.eu) a point-of care device for the early diagnosis of sepsis has been developed. The portable device is an optical microarray reader based on a novel design combining interferometric lens-free microscopy and CMOS image sensing. Gold nanostructures designed to increase the sensitivity of the device are used as sensing chips. Accurate surface modification and biofunctionalization protocols have been developed for the optimal immobilization of specific receptors (both antibodies and DNA probes) for a panel of selected biomarkers (Several proteins, miRNAs and bacteria). Individual detection assays in a label-free microarray format have been developed and sensitivity levels established, to further integrate them in a unique chip that will allow the analysis of several biomarkers at the same time (multiplexed measurement).Horizon 2020 European project (RAIS, www.rais-project.eu).Peer reviewe