Novel and versatile approaches for DNA-based nanosensing based on nucleic acid triplex formation

Resumen del trabajo presentado al VIII International Congress on Analytical Nanoscience and Nanotechnology, celebrado en Barcelona (España) del 3 al 5 de julio de 2017.DNA is an ideal molecule for sensing not only because of its specificity but also because they can be prepared and modified with high efficiency and selectivity. DNA nanostructure-based sensors may provide highly specific detection based on the molecular recognition properties of DNA as well as the possibility of developing specific aptamers for biologically relevant molecules. Some time ago, our group reported the triplex-stabilizing properties of 8-aminopurines and the use of parallel and antiparallel tail clamps to increase the efficiency of triplex formation with structured DNA and RNA targets. This knowledge has been recently applied to develop novel DNA detection techniques based on the stability of triplex by using tail-clamps carrying 8-aminopurines as bioreceptor. In the present communication we will describe the optimization of these methods for the detection of bacterial mRNA as well as miRNAs.Peer Reviewe