Pore analysis of the lyophilisate\u27s microstructure

Liofilizacija je kompleksen proces sušenja z zamrzovanjem, s sublimacijo in desorpcijo. Produkt liofilizat je suha snov v obliki porozne pogače. Za analizo prenosa toplote in snovi pri liofilizaciji potrebujemo kakovostne podatke o velikosti, obliki in distribuciji por liofilizata. S programskim orodjem ImageJ smo izvedli analizo SEM slik površine pogače. Pri tem smo obravnavali velikost in razporeditve por. Uporabljeno orodje ImageJ je primerno za ročno obdelavo slik, s programskim določanjem mej por pa z obravnavanimi izhodiščnimi predpostavkami nismo uspeli doseči željene natančnosti vsled kompleksnih površin.Freeze-drying is a complex process of drying the solution with the use of freezing, sublimation and desorption. The product lyophilisate is a dry material in the shape of a porous cake. For the analysis of the heat and mass transfer during freeze-drying, quality data about the size, shape and distribution of lyophilisate\u27s pores is required. Analysis of the SEM pictures of the cake\u27s surface was performed with the use of ImageJ software. Size and the arrangement of the pores were taken into account. The used software, ImageJ, is appropriate for the manual treatment of pictures, while the desired accuracy, with the use of automatic determination of the pore borders with discussed baseline assumptions, was unable to be reached, mainly because of the surface complexity