Detection of aberrant transcription of major histocompatibility complex class II antigen presentation genes in chronic lymphocytic leukaemia identifies HLA-DOA mRNA as a prognostic factor for survival
- Souwer, Y
- Chamuleau, M.E.D.
- Loosdrecht, A.A. van de
- Tolosa, E.
- Jorritsma, T.
- Muris, J.J.F.
- Poppel, M.N.M. van
- Snel, S.N.
- Corput, L. van de
- Ossenkoppele, G.J.
- Meijer, C.J.L.M.
- Neefjes, J.J.
- Ham, S.M. van
DOIAbstract
In human B cells, effective major histocompatibility complex (MHC) class II-antigen presentation depends not only on MHC class II, but also on the invariant chain (CD74 or Ii), HLA-DM (DM) and HLA-DO (DO), the chaperones regulating the antigen loading process of MHC class II molecules. We analysed immediate ex vivo expression of HLA-DR (DR), CD74, DM and DO in B cell chronic lymphocytic leukaemia (B-CLL). Real-time reverse transcription polymerase chain reaction demonstrated a highly significant upregulation of DRA, CD74, DMB, DOA and DOB mRNA in purified malignant cells compared to B cells from healthy donors. The increased mRNA levels were not translated into enhanced protein levels but could reflect aberrant transcriptional regulation. I...
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