Nitrergic effects on evoked and spontaneous release require cpx.

<p>(A) Raw eEJC recordings of a 50-Hz train, 500 ms in cpx^-/- larvae (±NO). (B) Mean QC for genotypes indicated, showing the lack of NO effects in cpx^-/- larvae (<i>w</i>^<i>1118</i> Ctrl in grey from <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003611#pbio.2003611.g002" target="_blank">Fig 2</a>). (C) Cumulative QC for the same conditions as in (B). (D) Mean QC and vesicle pool sizes for the genotypes indicated (<i>w</i>^<i>1118</i> Ctrl data from Figs <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003611#pbio.2003611.g001" target="_blank">1</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003611#pbio.2003611.g002" target="_blank">2</a>, ****<i>p</i> < 0.0001 versus <i>w</i>^<i>1118</i> Ctrl). (E) mEJCs recordings from m6 and m5 in cpx^-/- and cpx^+/- ± NO. (F) Mean mEJC frequencies for conditions and genotypes indicated (****<i>p</i> < 0.0001 versus m5 <i>w</i>^<i>1118</i> Ctrl, #<i>p</i> < 0.05 versus m6 cpx^+/- Ctrl). (G) Raw traces of mEJC recordings before (left) and after (right) high frequency stimulation in <i>w</i>^<i>1118</i> (±NO) and NOS^C larvae. (H) Average fold change of mEJC frequency during 50 s after stimulation compared to baseline frequency before stimulation for conditions and genotypes indicated (NOS “null” comprised of data from NOS^C and NOS^Δ15). NO treatment (40 min) suppresses increases in frequency, NOS “null” potentiates relative increases and enhanced denitrosylation (GCLm and GSNOR OE) prevents NO-induced suppression (*<i>p</i> < 0.05, **<i>p</i> < 0.01 versus Ctrl, ##<i>p</i> < 0.01, ####<i>p</i> < 0.001 versus NO). The raw data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2003611#pbio.2003611.s015" target="_blank">S5 Data</a>. Data denote mean ± SEM in all graphs, ANOVA with post hoc Tukey-Kramer. Ctrl, control; cpx, complexin; eEJC, evoked EJC; EJC, excitatory junction current; GCLm, glutamate-cysteine ligase catalytic subunit M; GSNOR, S-nitrosoglutathione reductase; mEJC, miniature EJC; m5, muscle 5; m6, muscle 6; NO, nitric oxide; OE, overexpression; QC, quantal content.