Additional file 6: of Reproducible protocols for metagenomic analysis of human faecal phageomes

Figure S3. Effect of repeated freeze-thaw cycles on the composition of faecal phageomes and bacteriomes (continued from Fig. 3 in the main text). A and B, α-diversity of bacterial (16S) and viral populations in faecal samples subjected to up to 5 successive freeze-thaw cycles or a prolonged storage at − 80 °C. C and D, Spearman rank ecological distances (β-diversity) between aliquotes of the three faecal samples used in the freeze-thaw experiment, calculated for both bacterial 16S and viral datasets and visualised via NMDS; NMDS stress values were 9.274361e-05 (C) and 9.926038e-05 (D). E, Barplot of relative abundance of bacterial taxonomic groups at the genus level. (PDF 376 kb