Additional file 6: of Osteoclasts degrade bone and cartilage knee joint compartments through different resorption processes

Figure S6. Resorption biomarkers released from osteoclasts cultured on osteochondral ECM in the pilot trial. Osteoclasts were cultured on osteochondral ECM from bovine femoral condyles in the presence or absence of resorption inhibitors. Resorption of calcified ECM and collagen type I was assessed by measuring the Ca2+ (A) and CTX-I (B) concentrations, respectively, in the medium. TRAP activity data could not be generated in these samples. Resorption of collagen type II was assessed by measuring C2M (C) concentrations in the medium. Data are presented as percent of vehicle with error bars representing the SEM. Statistical significance is indicated by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for comparisons against the vehicle; the post hoc test used is indicated in the top left corner of each graph. (PDF 27 kb