Effects of melatonin on sFLT and sENG secretion.

<p>Placental explant tissue, primary cytotrophoblasts and human umbilical vein endothelial cells (HUVECs) were treated with melatonin (100–1000μM placental explant tissue and 1–1000μM for isolated cells) for 48 h. Placental explant secretion of sFLT (A) and sENG (B) was assessed following melatonin treatment. Cytotrophoblast secretion of sFLT (C) following melatonin. HUVEC secretion of sFLT (D) and sENG (E) with increasing doses of melatonin. Experiments were performed with technical triplicates and all experiments were repeated a minimum of three times. Data is expressed as mean % change from control ± SEM. All data were analyzed by Kruskal-Wallis followed by Dunn’s multiple comparisons test (***p≤ 0.001).