Comparison of cross-reactivity of serum IgG from influenza virus infected versus Addavax adjuvanted rHA vaccinated mice.

<p>(<b>A</b>) Multiplex assay comparison of IgG seroreactivity against a panel of 29 influenza rHA resulting from viral infection or the prime-boost-boost vaccination with Addavax (MF59-like) admixed rHAs from either A/California/07/2009 (A/Cal09, H1N1) or A/HongKong/1/1968 (A/HK68, H3N2). Sera were collected 9 weeks post- infection, or 3 weeks after the final vaccine boost (day 63 after the priming vaccination). Sera harvested from six mice per group were pooled for analysis. The IgG binding against influenza HA are reported as the mean MFI minus the baseline of each strain (n = 3). (<b>B</b>) A cross-reactivity plot of the mouse serum IgG binding data for each influenza virus strain versus the protein feature dissimilarity of vaccine HA and each of the rHA proteins used in mPlex-Flu assay.