<p>(A) CD45RO. (B) CD105. (C) STRO-1. (D) The arrows indicate the perinuclear location of Nanog. (E) The arrows indicate the location of RANKL. (F) Levels of expression of markers. Cell images showing the expression of the markers CD 45RO, RANKL, Nanog, CD105, and STRO-1 in osteoblasts cultured in osteogenic medium for 16 days. Data are expressed as mean ± standard deviation. An ANOVA followed by Tukey’s test was used to analyze the data. P < 0.005.</p
<p>Gene expression analysis through qRT-PCR of the osteoblast and osteoclast markers with regards to...
grantor: University of TorontoA challenge in analyzing transitional stages in the osteobla...
Dot plot graphs illustrate the presence/absence of mesenchymal cell surface markers, performed by fl...
<p>A) The expression of alkaline phosphatase (ALP), an early stage maker gene of osteoblast differen...
<p>(A) Micrographs of MSCs treated with osteogenic media for 0, 3, 9, 15 and 24 days. (Scale bar: 10...
<p>(A) hASCs used as the negative controls. (B) Image of cells cultured for 7 days, showing a less i...
<p>(A) Cells were cultured in an osteogenic medium for 7, 16, or 21 days to RANKL gene. RANKL genes ...
Total RNA was isolated from triplicate cultures of MC3T3-E1 cells at days 2, 5, 10 and 28 in culture...
<p>As markers of differentiation we analyzed the expression of bone-related genes, BSP (A), ALP (B) ...
<p>(a) Osteogenic differentiation was confirmed by Alizarin Red staining after 40 days in OBM. The u...
<p>The temporal expression patterns of the osteocyte marker genes, <i>E11</i> (A), <i>Dmp1</i> (B), ...
Today, the tool that is most commonly used to evaluate the osteogenic differentiation of bone marrow...
<p>A: C4-2B and PC-3 cells were treated with 100 ng/ml OPG or 10 ng/ml TGF-β for 24 hours. The expre...
<p>(A–D) Cultured were stained with Alizarin Red S after 3 weeks to detect osteogenic induction. (A,...
<p>A. The cell attachment on Vitoss™ after seeding with aspirates of IC-BM (left panel) and VB-BM (r...
<p>Gene expression analysis through qRT-PCR of the osteoblast and osteoclast markers with regards to...
grantor: University of TorontoA challenge in analyzing transitional stages in the osteobla...
Dot plot graphs illustrate the presence/absence of mesenchymal cell surface markers, performed by fl...
<p>A) The expression of alkaline phosphatase (ALP), an early stage maker gene of osteoblast differen...
<p>(A) Micrographs of MSCs treated with osteogenic media for 0, 3, 9, 15 and 24 days. (Scale bar: 10...
<p>(A) hASCs used as the negative controls. (B) Image of cells cultured for 7 days, showing a less i...
<p>(A) Cells were cultured in an osteogenic medium for 7, 16, or 21 days to RANKL gene. RANKL genes ...
Total RNA was isolated from triplicate cultures of MC3T3-E1 cells at days 2, 5, 10 and 28 in culture...
<p>As markers of differentiation we analyzed the expression of bone-related genes, BSP (A), ALP (B) ...
<p>(a) Osteogenic differentiation was confirmed by Alizarin Red staining after 40 days in OBM. The u...
<p>The temporal expression patterns of the osteocyte marker genes, <i>E11</i> (A), <i>Dmp1</i> (B), ...
Today, the tool that is most commonly used to evaluate the osteogenic differentiation of bone marrow...
<p>A: C4-2B and PC-3 cells were treated with 100 ng/ml OPG or 10 ng/ml TGF-β for 24 hours. The expre...
<p>(A–D) Cultured were stained with Alizarin Red S after 3 weeks to detect osteogenic induction. (A,...
<p>A. The cell attachment on Vitoss™ after seeding with aspirates of IC-BM (left panel) and VB-BM (r...
<p>Gene expression analysis through qRT-PCR of the osteoblast and osteoclast markers with regards to...
grantor: University of TorontoA challenge in analyzing transitional stages in the osteobla...
Dot plot graphs illustrate the presence/absence of mesenchymal cell surface markers, performed by fl...