Fitness consequences of evolving Cit⁺ in different evolved genetic backgrounds.

<p><b>(a)</b> Results of the P_<i>rnk</i><i>-citT</i> knock-in assay on 23 pre-Cit⁺ evolved strains. The clones are ordered by the generation from which they were isolated. Error bars are 95% confidence intervals. Strain construction details and how the results of competition assays were combined into these fitness estimates are described in the <b>Methods</b> and <b><a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007348#pgen.1007348.s001" target="_blank">S1</a>–<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007348#pgen.1007348.s004" target="_blank">S4</a> Figs</b>. <b>(b)</b> Increased lag phase upon addition of the P_<i>rnk</i>-<i>citT</i> module in anti-potentiated strains. Growth curves for the ancestor, REL606, and two anti-potentiated strains, ZDB483 and ZDB14, are shown. Error bars are standard deviations of four replicate cultures.