SAS sensitizes B16F10 cells to X-irradiation.

<p>A colony formation assay was used to determine the cytotoxicity and the radio-sensitizing effect of SAS treatment (24 h). (A) Colony formation assay for treatment with SAS alone. B16F10 cells were seeded and treated with SAS at the concentrations shown. (B) Colony formation assay for treatment with SAS plus X-irradiation. After pretreatment with SAS (200 μM, 24 h), B16F10 cells were subjected to X-irradiation at the doses shown. (C) Evaluation of MC fraction after X-irradiation. B16F10 cells were stained for γ-tubulin after 8 Gy of irradiation with or without SAS treatment. IR, X-irradiation. (D) Quantification of MC fractions after manual counting. IR, irradiation, error bars = SD, *p<0.05.