Analysis of ribotype 591 by MLVA.

<p>MLVA was applied to 27 isolates belonging to the ribotype 591. Dendrogram showing similarities of banding patterns generated with high-resolution capillary gel-based electrophoresis (CE)-PCR. MLVA clusters are identified by minimum-spanning tree: one clonal complex is defined by a MLVA type with less than a two-locus difference. In addition, free fecal toxin A/B (FFT), toxin in cell culture (TC), presence of PaLoc genes (<i>tcd</i>A/<i>tcd</i>B), minimal inhibitory concentrations (MIC) for vancomycin (VAN), metronidazole (MTZ), moxifloxacin (MOX) and clindamycin (CLIN) are shown for each isolate with the respective breakpoints. VNTR, variable-number tandem repeat.