Effect of RNAi on <i>LsLpR</i> transcript and lipid levels in copepodids.

<p>(A) Relative Expression of <i>LsLpR</i> in the copepodids (7 dph) after knock downed in nauplius larva. Error bars show standard deviation. Asterisk represents significant difference (independent-samples T-test, p < 0.05) in mRNA levels of <i>LsLpR</i> between the control group (n = 5) and the knock-down group (n = 5). (B-D) Detection of neutral lipids by Oil Red O stain. Lipid contents in the copepodids hatched from <i>LsLpR</i> (fragments 1 + 3) (B) and control dsRNAs treated nauplii (C). Semi-quantification of total neutral lipids with Oil Red O stain in copepodids (n = 5, each replicate contains 25 animals) developed from nauplii treated with control and <i>LsLpR</i> dsRNAs (fragments 1 + 3) (D). No significant difference (independent-samples T-test, p > 0.05) was found between control group and <i>LsLpR</i> dsRNA-treated group. Scale bars = (B-C) 1 mm.