Treatment with dsRNA against <i>LsLpR</i>.

<p>(A) Relative expression of <i>LsLpR</i> in the adult females after injection of dsRNA in pre-adult females (30–32 days post injection). (B) Relative expression of <i>LsLpR</i> after injection of dsRNA (fragment 1 + 3) in adult females and measured at days 5, 10 and 15 (post injection). Expression PCR was carried out on 5 female lice from control and knock-down group at each time point. (C) Relative expression of <i>LsLpR</i> in copepodids (n = 5 × 20) after knock down (fragments 1 + 3) in nauplii I, assayed before the infection of a host and in adult female lice at the time of termination of the experiment. Error bars show standard deviation and P-values for independent-samples T-test analysis are shown, expression levels of LsLpR in control versus LsLpR dsRNA-treated group.