Quantification of the gene candidates likely involved in ChA biosynthesis by qRT-PCR.

<p><b>(A)</b> Relative expression levels by qRT-PCR analysis of six key genes, chosen from the RNA-seq profiling and found in the chaetoglobosin biosynthetic gene cluster, in the transformants compared with the wild-type (WT) strain. The six genes are C6 zinc finger protein (CHGG_01237), transposase (CHGG_01238), PKS−NRPS hybrid gene cluster, <i>CgcheA</i> (CHGG_01239), P450 oxygenase (CHGG_012421), FAD-dependent oxidoreductase (CHGG_012422) and P450 oxygenase (CHGG_01243). All transcripts were normalized against <i>actin</i> cDNA amplified with primers qActin(s) and qActin(as) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0195553#pone.0195553.s001" target="_blank">S1 Appendix</a>). <b>(B)</b> Decreased expression levels of <i>CglaeA</i> in pG14 and restoration in pGP6. There is significantly difference between the mutant and wild-type as indicated by two asterisks (p-value <0.001 with T-test analysis), ns: no significant difference. Experiments were performed in triplicate.