Kinetic model development and monitoring of specific antibiotic degradation during the wastewater process

Magistrsko delo prikazuje optimizacijo procesov čiščenja odpadnih vod pri proizvodnji antibiotikov. Na podlagi analiz reakcij, izvedenih v laboratorijskem merilu, so podani ocena stanja trenutnega procesa in predlogi izboljšav. V prvi fazi raziskav smo v manjšem reaktorju izvedli serijo eksperimentov na pripravljenih vzorcih vod in z analizo na HPLC za namene kinetskih študij, določili koncentracijske profile ključnih substanc – amoksicilina in klavulanske kisline. Ugotovili smo, da za razpad klavulanske kisline ne moremo določiti kinetike, saj je reakcija prehitra in koncentracija kisline preide v območje pod mejo zaznave izbrane analitske metode. S pomočjo standardov smo določili linearno območje koncentracij, ki je za amoksicilin od (20-200) µg/mL. V drugi fazi raziskav smo s spreminjanjem določenih procesnih parametrov izvedli optimizacijo reakcije nevtralizacije, ki je del obstoječega procesa čiščenja odpadne vode. Cilj magistrske naloge je bil doseči najnižjo možno koncentracijo amoksicilina po koncu nevtralizacije, kar nam je uspelo pri T = 30 °C in pH = 11. Raziskave smo zaključili z analizami realnih vzorcev vtočne in iztočne vode čistilne naprave.The master\u27s thesis presents the optimization of wastewater treatment processes in the production of antibiotics. Based on the analysis of reactions carried out on a laboratory scale, an assessment of the status of the current process and suggestions for improvements are given. In the first phase of the research, a series of experiments were carried out on the prepared water samples in a small reactor, and by determining the concentration profiles of the key substances - amoxicillin and clavulanic acid by HPLC analysis for the purposes of kinetic studies. We have found that clavulanic acid must not be used for the purposes of kinetic studies, as it rapidly decays during the reaction and thus passes into the area below the detection limit of the selected analytical method. Using the standards, a linear range was determined for amoxicillin from (20-200) μg / mL. In the second phase of the research, the optimization of the neutralization reaction, which is part of the existing wastewater treatment process, was performed by changing certain process parameters. The objective of the master\u27s thesis was to achieve the lowest possible concentration of amoxicillin after the end of neutralization, which we succeeded at T = 30 ° C and pH = 11. The research was completed by analyzing the real samples of the inlet and outlet water of the treatment plant