Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules

Neutron irradiation Of Cp2MoCl2 for 24 h afforded the radiotracer (CP2MoCl2)-Mo-99 which was characterised by UV-Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for Mo-99 using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction Of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA. (c) 2006 Elsevier Ltd. All rights reserved