Isolation and characterization of chromium reducing Vibrio sinaloensis from marine soil

This project report is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016.Cataloged from PDF version of project report.Includes bibliographical references.Now-a-days, hexavalent chromium is one of the most toxic environmental pollutants which is very toxic transportable and also is used in almost every industries and released in the similar manner. Reduction of the hexavalent chromium to its water- insoluble trivalent chromium makes them less toxic as their bioavailability decreases. Recently, this particular matter has acknowledged specific attention for their effective use in bioremediation process. To isolate, characterize and identify the bacteria, samples were collected from the coastal regions of Bangladesh situated in Ship Breaking Yard of Sitakunda, which are capable of reducing hexavalent chromium to trivalent chromium. The isolated organism has been studied for chromium (VI) reduction activity for their dependence on growth. The isolate was resistant to hexavalent chromium and also had reducing activity. The isolate S2 showed the optimum reduction activity at 370C temperature and both in pH 7 and 8.5. Finally, cultural and biochemical tests of the isolate were carried out for identification of bacteria. After analyzing the biochemical test data in Advanced Bacterial Identification System Version 6 (ABIS 6.0), the isolate was identified as Vibrio sinaloensis.Mehmuna MorshedB. Pharmac