A new method for yeast phagocytosis analysis by flow cytometry

Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved.This research was supported by FEDER Funds through the Operational Programme COMPETE and national funds through the Fundação para a Ciência e Tecnologia (FCT), in the scope of project PEst-OE/BIA/ UI4050/2014.