A GEFI collaborative exercise on DNA/RNA co-analysis and mRNA profiling interpretation
- Carnevali, E.
- Lacerenza, D.
- Severini, S.
- Alessandrini, F.
- Bini, C.
- Di Nunzio, C.
- Di Nunzio, M.
- Fabbri, M.
- Fattorini, P.
- Piccinini, A.
- Ponzano, E.
- Portera, G.
- Previder\ue3, C.
- Renieri, A.
- Scarnicci, F.
- Verzeletti, A.
- Pelotti, S.
- van den Berge, M.
- Sijen, T.
- Robino, C.
DOIAbstract
A collaborative exercise on DNA/RNA co-analysis and RNA cell typing involving 15 GEFI (Italian working group of ISFG) laboratories was organized in collaboration with the Netherlands Forensic Institute. Participants received: 1) PCR primers for a 19-plex mRNA profiling assay, with reference purified PCR products for each cell type targeted in the multiplex; 2) detailed protocols for DNA/RNA co-extraction, mRNA profiling, and interpretation of results; 3) a set of 8 mock forensic stains (7 single source, one a mixture of two body fluids). All but one laboratory generated correct DNA typing results. As expected, stochastic effects were seen for low template DNA extracted from a skin stain. As for mRNA profiling, the percentage of laboratories...
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