The Ways of Factors Influencing High-Solid Enzymatic Hydrolysis of Sugarcane Bagasse Treated by Liquid Hot Water

The influence ways of structural feature, hydrolytic viscosity and monosaccharides concentrations on the enzymatic hydrolysis of high-solid liquid hot water (LHW)-treated lignocellulose were evaluated. The SEM observation showed that the residual lignin in the LHW-treated sugarcane bagasse (SCB) played an important role in gluing fasciculate structures, and XRD detection indicated the decrease of crystalline index (CrI) of LHW-treated SCB after lignin removal. The enzymatic hydrolysis of LHW-treated SCB with and without lignin removal showed that the residual lignin might block the access of cellobiohydrolase to the crystalline cellulose. The fractal kinetic model indicated that the benefit of the low viscosity for high-solid enzymatic hydrolysis of LHW-treated SCB would appear with the time going. As the enzymatic hydrolysis proceeding, the accumulation of glucose imposed more and more intense inhibition on the cellulolytic hydrolysis, while the xylose showed a little inhibition. The changes of UV spectra indicated that the glucose accumulation hardly affected the side chains containing tyrosine (Try) and/or tryptophan (Trp) which should be not in the catalytic active center of cellulase