Early changes in eNOS and ICAM-1 expression in mice aorta after administration of high fat diet.

Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Consultant: Associate Professor, Petr Nachtigal, Ph.D. Candidate: Tereza Stehlíková Title of Thesis: Early changes in eNOS and ICAM-1 expression in mice aorta after administration of high fat diet Background: The aim of this thesis was to observe changes in eNOS a ICAM-1 expression after administration of high fat diet in comparison with the control group, which was fed with the chow diet. Method: C57BL/6J female mice were used for the experiment. 6 mice were fed with the high fat diet (experimental group), which contains 1,25 % of cholesterol and 40 % of fat, for the period of 3 months. Six mice (representing control group) were fed with the chow diet the whole time. Immunohistochemical analysis was performed by means of avidin-biotin complex method. Results: The expression of eNOS was visible on the vascular endothelium but there were no differences between the control group and the experimental group. Similarly, ICAM-1 expression was detected only in endothelium in aorta but no differences in the expression between control and experimental group were visible. Conclusions: Administration of high fat diet with 1,25 % of cholesterol and 40 % of fat for 3 months had no effect on the expression of..