Optimization approach to the detection of sentinel node in endometrial cancer

Title: "Optimization Approach to the Detection of Sentinel Node in Endometrial Cancer." Design: prospective experimental study Aim of thesis : The purpose of this study was to evaluate predictive value of the in vivo sentinel lymphnode (SLN) mapping in patientswithendometrialcancer and verifyamodified method of the application of subserosal blue dye by laparotomy versus laparoscopy approach. Detection substance is applied subserosally in the uterine edges vicinity the round ligament of uterus and uterine vessels in the isthmic portion of the uterus. Detection rate of the SLN was endpoint of this study. Presence of nodular adenomyosis in uterine specimens with endometrial cancer was another endpoint. The findings were compared with our retrospective study on malignant changes in adenomyosis in women with endometrial cancer. Materials and methods: Altogether thirty-one patients were divided into two groups. Group A - patients with intermediate and high risk endometrial cancer stages I were subjected to staging laparotomy with intraoperative detection of SLN and subsequent completion of the pelvic and paraaortic lymphadenectomy. Harvested SLN was routinely examined by classical haematoxilin eosin staining and in case of negativity, immunohistochemistry with antikeratine antibodies AE1/AE3 was applied...