Otter qPCR Data at SAFE

<b>Description: </b><p>All data collected during my project at the SAFE project, March-April 2017. This document contains metadata that should summarise all data collected.</p><p><b>Project: </b>This dataset was collected as part of the following SAFE research project: <a href="https://www.safeproject.net/projects/project_view/166"><b>Using environmental DNA (eDNA) as a tool for monitoring the biodiversity of tropical otter species</b></a></p><p><b>XML metadata: </b>GEMINI compliant metadata for this dataset is available <a href="https://www.safeproject.net/datasets/xml_metadata?id=13">here</a></p><p><b>Data worksheets: </b>There are 2 data worksheets in this dataset:</p><ol><li><p><b>eDNA</b> (Worksheet eDNA)</p><p>Dimensions: 66 rows by 33 columns</p><p>Description: eDNA' contains all data related to the qPCR elements of this project</p><p>Fields: </p><ul><li><b>Date</b>: Date Collected (Field type: Date)</li><li><b>Session</b>: Morning or Evening (Field type: Categorical)</li><li><b>Site</b>: Catchment Site; indicates metres upriver from SAFE Project hydrology datalogger (Field type: ID)</li><li><b>Code</b>: Unique Code given to each samplea and subsample (1a, 1b, 1c…) (Field type: ID)</li><li><b>Location</b>: Riparian transect sample collected from (Field type: Location)</li><li><b>Time_Start</b>: Time arrived at site (Field type: Time)</li><li><b>Time_Finish</b>: Time left site (Field type: Time)</li><li><b>eDNA_Start</b>: Sample collection starting time (Field type: Time)</li><li><b>eDNA_Stop</b>: Sample collection ending time (Field type: Time)</li><li><b>T_w</b>: Water Temperature at time of sample (Field type: Numeric)</li><li><b>T_a</b>: Air Temperature at time of sample (Field type: Numeric)</li><li><b>pH</b>: pH of surface water (Field type: Numeric)</li><li><b>R_H</b>: Relative humidty at site (Field type: Numeric)</li><li><b>Lux</b>: Lux score at time of sample (Field type: Numeric)</li><li><b>Precip</b>: Precipitation Present (Yes or No) (Field type: Categorical)</li><li><b>Shade</b>: Presence of shade during collection (Field type: Categorical)</li><li><b>Leaf_Litter</b>: Presence of leaf litter during collection (Field type: Categorical)</li><li><b>Substrate</b>: Type of substrate (Field type: Categorical)</li><li><b>Time1</b>: Time taken for "bottle" to travel River_dist; used to calculate flow rate (Field type: Numeric)</li><li><b>Time2</b>: Time taken for "bottle" to travel River_dist; used to calculate flow rate (Field type: Numeric)</li><li><b>Time3</b>: Time taken for "bottle" to travel River_dist; used to calculate flow rate (Field type: Numeric)</li><li><b>TimeAv</b>: Average time taken for "bottle" to travel River_dist; used to calculate flow rate (Field type: Numeric)</li><li><b>Depth1</b>: Depth of sample (Field type: Numeric)</li><li><b>Depth2</b>: Depth of sample (Field type: Numeric)</li><li><b>Depth3</b>: Depth of sample (Field type: Numeric)</li><li><b>DepthAv</b>: Average_depth of sample (Field type: Numeric)</li><li><b>River_Dist</b>: Distance "bottle" travelled at sample site ; used to calculate flow rate (Field type: Numeric)</li><li><b>Flow</b>: Calculated flow (speed = distance/ time) (Field type: Numeric)</li><li><b>Presence</b>: Presence of otters detected (to a level deemed above background noise on qPCR) (Field type: Categorical)</li><li><b>N. Wells Pos</b>: Presence of otters detected (to a level deemed above background noise on qPCR) (Field type: Abundance)</li><li><b>Positive NTC</b>: Number of positive negative controls out of 12 (Field type: Numeric)</li><li><b>Notes</b>: Any important notes during sampling (Field type: Comments)</li></ul><br></li><li><p><b>Traditional</b> (Worksheet Traditional)</p><p>Dimensions: 122 rows by 12 columns</p><p>Description: Traditional' contains data collected using traditional surveys</p><p>Fields: </p><ul><li><b>Location</b>: Riparian transect sample collected from (Field type: Location)</li><li><b>Session</b>: Morning or Evening (Field type: Categorical)</li><li><b>Date</b>: Date of sampling (Field type: Date)</li><li><b>Time</b>: Time of sampling (Field type: Time)</li><li><b>Site</b>: Catchment Site; indicates metres upriver from SAFE Project hydrology datalogger (Field type: ID)</li><li><b>Otter</b>: Otter observed at site? 0=no, 1=yes (Field type: Abundance)</li><li><b>Spraint</b>: Otter faeces at site? 0=no, 1=yes (Field type: Abundance)</li><li><b>Den</b>: Den present at site? 0=no, 1=yes (Field type: Abundance)</li><li><b>Footprints</b>: Footprints at site? 0=no, 1=yes (Field type: Abundance)</li><li><b>Remains</b>: Remains of prey present? 0=no, 1=yes (Field type: Abundance)</li><li><b>Notes </b>: Any additional notes deemed important (Field type: Comments)</li></ul><br></li></ol><p><b>Date range: </b>2017-03-14 to 2017-04-11</p><p><b>Latitudinal extent: </b>4.6508 to 4.7255</p><p><b>Longitudinal extent: </b>117.5765 to 117.5980</p><p><b>Taxonomic coverage: </b><br> All taxon names are validated against the GBIF backbone taxonomy. If a dataset uses a synonym, the accepted usage is shown followed by the dataset usage in brackets. Taxa that cannot be validated, including new species and other unknown taxa, morphospecies, functional groups and taxonomic levels not used in the GBIF backbone are shown in square brackets.</p><div>Animalia<br> - Chordata<br> - - Mammalia<br> - - - Carnivora<br> - - - - Mustelidae<br></div><p>