Expression of genes of interest at ZT8 and ZT20.

<p><i>A</i>: Microarray data (GSE109882) unraveled a total of 301 transcripts significantly up or downregulated (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193882#pone.0193882.s003" target="_blank">S1 Table</a> for full details). 18 genes of interest (note that hemoglobin alpha and beta–Hba and Hbb- bore two probes on the microarray chip). Genes have been grouped by function. $ denotes genes identified in ref [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193882#pone.0193882.ref014" target="_blank">14</a>]. N = 5 experimental replicates for each group. B: relative expression of <i>glucagon</i>, <i>Ins1</i>, <i>Ins2</i>, <i>Cav1</i>.<i>2</i> and <i>Cav1</i>.<i>3</i> at ZT8 and ZT20 measured by qRT-PCR. Data are from 3 animals with 2 technical replicates per animal and per ZT.