Chromatin immunoprecipitation (ChIP) method for non-model fruit flies (Diptera: Tephritidae) and evidence of histone modifications - Fig 2

<p>(A) An example of product size after sonication using Bioruptor at 30 sec on, 30 sec off at high speed for 70–80 minutes; (B) Conjugation of (a) H3K27me3 (b) H3K27 acetylation (c) mock in <i>B</i>. <i>tryoni</i> checked for <i>Obp 99c</i> and <i>Unigene266</i> genes; (C) Bioanalyzer image showing the size and concentration of chromatin DNA immunoprecipitated with H3K27me3 (D) Chromatin DNA immunoprecipitated with H3K27me3 after enrichment.