Expression of <i>M</i>. <i>incognita</i> profilin genes <i>MiPFN1</i> and <i>MiPFN3</i> in <i>M</i>. <i>incognita</i> at different life stages.

<p>The relative expression of (A) <i>MiPFN1</i> and (B) <i>MiPFN3</i> in eggs, J2, and nematodes inside tomato tissues at 7, 14, and 21 days post-inoculation was determined by Real-Time qRT-PCR. The relative expression of (C) <i>MiPFN1</i> and (D) <i>MiPFN3</i> in eggs, J2, and infective Arabidopsis roots at 4, 7, 14, and 42 dpi was also determined by Real-Time qRT-PCR. The fold change values were calculated using the 2-ΔΔCt method using reference gene <i>MiGAPDH</i>. Data presented as the change in expression relative to that of egg. The mean values (± SD) of the fold change for three biological replicates. *, <i>P</i><0.05, One sample t-test.