Analysis of miR-203-3p and IL-33 expression in the liver during infection.

<p>(A, B) The expression of miR-203-3p (A) and <i>Il-33</i> mRNA (B) in the livers during infection was detected by qPCR. * <i>P</i><0.05, *** <i>P</i><0.001, compared with samples of day 0. (C, D) The expression of miR-203-3p (C) and <i>Il-33</i> mRNA (D) in different types of primary liver cells during infection was detected by qPCR. *** or ### <i>P</i><0.001, compared with samples of day 0. (E, F) Primary hepatocyte, HSCs, and Kupffer cells (KCs) were isolated from uninfected and infected (day 42 post-infection) livers, and the levels of miR-203-3p (E) or <i>Il33</i> mRNA (F) in HSCs and KCs were compared to those in hepatocytes from uninfected livers. (G) The expression of IL-33 in different types of primary liver cells from uninfected mice or infected (day 42 post-infection) mice was detected by western blot. (H) IL-33 (red) colocalization with α-SMA (green) in the infected (day 42 post-infection) liver sections was detected by immunohistochemistry. Cells staining positive for both IL-33 and α-SMA are indicated with arrows. The right picture is an amplified part of the left picture. Data are expressed as the mean ± s.d. from three independent experiments. Multiple comparisons were performed by one-way ANOVA, and followed by Bonferroni post test for comparison between two groups.