Additional file 3: of High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers

Figure S1. SDS-PAGE analyses of the different steps of the downstream purification processes of the SacPox (a) and SsoPox 3M (b) enzymes from biomasses of fed-batch fermentation induced with 10.0 mM galactose: lane 1-ladder, lane 2-crude extract, lane 3-thermal precipitated sample, lane 4-retentate on 100 kDa, lane 5-permeate on 100 kDa, lane 6-retentate on 5 kDa; lane 7-permeate on 5 kDa; lane 8-immunoblotting of retentate on 5 kDa with specific antibodies. Percentage of representativity of the SacPox and SsoPox 3M enzyme bands in the different purification steps (c). (PDF 309 kb