Glucagon effect on body weight and food intake.

<p>Top panel: the change in body weight after twice daily dosing for two days with insulin alone or four days in combination with the LAG. A) 86 nmol/kg insulin ± 1nmol/kg LAG. B) 120 nmol/ kg insulin ± 1nmol/kg LAG. Data are expressed as means ± SEM; n = 20. A significant change in body weight compared to insulin alone is indicated by ** (p = 0.002) and **** (p < 0.0001) asterisks (analyzed using two-tailed paired t-test). Bottom panel: The average food intake per day per animal during insulin treatment alone or in combination with the LAG. C) 86 nmol/kg insulin ± 1nmol/kg LAG. D) 120 nmol/ kg insulin ± 1nmol/kg LAG. Data are expressed as means of 2 days ± SEM; n = 10. A significant effect of adding 1 nmol/kg LAG to 120 nmol/kg insulin is indicated by ** (p = 0.001) (analyzed using a two-tailed paired t-test).