Impact of <i>Ureaplasma</i> co-stimulation on LPS-induced MCP-1 and MCP-3 responses in neonatal and adult monocytes.

<p>Using qRT-PCR and multi-analyte immunoassay, we assessed mRNA expression (A, B) and protein secretion (C, D) in LPS-primed monocytes co-stimulated with <i>Ureaplasma</i> isolates. LPS-primed monocytes exposed to <i>Ureaplasma</i> broth served as negative control, LPS-activated cells served as positive control. Values represent the means ± SD (^# <i>p</i> < 0.05, vs. LPS-primed monocytes exposed to broth control; ^○ <i>p</i> < 0.05, vs. LPS-activated monocytes).