MOESM6 of Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis

Additional file 6: Figure S3. Profiling of the expression of SvCAD3, SvCAD5, SvCAD6 and SvCAD7 following normalisation with the most suitable, and the least suitable, set of reference genes. A–D RT-qPCR data to profile the expression of SvCADs, SvCAD3 (A; Sevir.6G025000), SvCAD5 (B; Sevir6G024400), SvCAD6 (C; Sevir7G014100), and SvCAD7 (D; Sevir7G245600) was normalised using the set of the three most suitable reference genes (ASPR6, DUSP and PP2A) and with the set of the three least suitable reference genes (FBoxD, PGM and SEIPIN)